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Ajouter au panierHardcover. Etat : Very Good. No Jacket. May have limited writing in cover pages. Pages are unmarked. ~ ThriftBooks: Read More, Spend Less.
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Ajouter au panierEtat : Good. Your purchase helps support Sri Lankan Children's Charity 'The Rainbow Centre'. Ex-library, so some stamps and wear, but in good overall condition. Our donations to The Rainbow Centre have helped provide an education and a safe haven to hundreds of children who live in appalling conditions.
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Ajouter au panierhardcover. Etat : New. In shrink wrap. Looks like an interesting title!
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Ajouter au panierEtat : New. Editor(s): Arnold, Frances H.; Georgiou, George. Series: Methods in Molecular Biology. Num Pages: 383 pages, biography. BIC Classification: PSF. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 20. Weight in Grams: 534. . 2010. Softcover reprint of hardcover 1st ed. 2003. Paperback. . . . .
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Ajouter au panierTaschenbuch. Etat : Neu. Directed Enzyme Evolution | Screening and Selection Methods | George Georgiou (u. a.) | Taschenbuch | xv | Englisch | 2010 | Humana Press | EAN 9781617374722 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.
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Ajouter au panierHardcover. Etat : Brand New. 1st edition. 383 pages. 9.00x6.25x1.25 inches. In Stock.
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Ajouter au panierEtat : New. Editor(s): Arnold, Frances H.; Georgiou, George. Series: Methods in Molecular Biology. Num Pages: 383 pages, biography. BIC Classification: PSF. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 20. Weight in Grams: 534. . 2010. Softcover reprint of hardcover 1st ed. 2003. Paperback. . . . . Books ship from the US and Ireland.
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Ajouter au panierPaperback / softback. Etat : New. This item is printed on demand. New copy - Usually dispatched within 5-9 working days.
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Ajouter au panierBuch. Etat : Neu. Directed Enzyme Evolution | Screening and Selection Methods | George Georgiou (u. a.) | Buch | Einband - fest (Hardcover) | Englisch | 2003 | Humana Press | EAN 9781588292865 | Verantwortliche Person für die EU: Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, productsafety[at]springernature[dot]com | Anbieter: preigu Print on Demand.
Langue: anglais
Edité par Humana Press, Humana Press, 2010
ISBN 10 : 1617374725 ISBN 13 : 9781617374722
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Ajouter au panierTaschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal to noise) to allow the isolation of lower activity clones early in evolution; (3) sufficiently reproducible to allow one to find small improvements; (4) robust, which means that the signal afforded by active clones is not dependent on difficult-to-control environmental variables; and, most importantly, (5) sensitive to the desired function. Regarding this last point, almost anyone who has attempted a directed evolution experiment has learned firsthand the truth of the dictum 'you get what you screen for. ' The protocols in Directed Enzyme Evolution describe a series of detailed p- cedures of proven utility for directed evolution purposes. The volume begins with several selection strategies for enzyme evolution and continues with assay methods that can be used to screen enzyme libraries. Genetic selections offer the advantage that functional proteins can be isolated from very large libraries s- ply by growing a population of cells under selective conditions.
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Ajouter au panierBuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal to noise) to allow the isolation of lower activity clones early in evolution; (3) sufficiently reproducible to allow one to find small improvements; (4) robust, which means that the signal afforded by active clones is not dependent on difficult-to-control environmental variables; and, most importantly, (5) sensitive to the desired function. Regarding this last point, almost anyone who has attempted a directed evolution experiment has learned firsthand the truth of the dictum 'you get what you screen for. ' The protocols in Directed Enzyme Evolution describe a series of detailed p- cedures of proven utility for directed evolution purposes. The volume begins with several selection strategies for enzyme evolution and continues with assay methods that can be used to screen enzyme libraries. Genetic selections offer the advantage that functional proteins can be isolated from very large libraries s- ply by growing a population of cells under selective conditions.