Aspöck andreas (11 résultats)

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Taschenbuch. Etat : Neu. Transient Transfection in Animal Cell Culture | A comparative study of different methods for transient transfection in suspension adapted Chinese Hamster Ovary cells | Andreas Aspöck | Taschenbuch | Einband - flex.(Paperback) | Englisch | 2010 | VDM Verlag Dr. Müller | EAN 9783639230888 | Verantwortliche Person für die EU: OmniScriptum GmbH & Co. KG, Bahnhofstr. 28, 66111 Saarbrücken, info[at]akademikerverlag[dot]de | Anbieter: preigu.…

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Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - The transient gene expression (TGE) in mammalian cells is an attractive option to rapidly produce research grade material with high quality that, for instance, can streamline the identification of candidate proteins in clinical trials. In this thesis we report on the performance of different methods to transiently transfect mammalian cells, in particular Chinese Hamster Ovary (CHO) cells. The used transfection methods can be categorized into physical and chemical. We compared the feasibility, the efficiency (both cost efficiency and transfection efficiency) and suitability regarding to up-scale the procedure. The optimization of the different transfection methods was carried out using statistical approaches. In this work, the successful optimization of the transient transfection and the up-scale into the bioreactor are reported. Furthermore a good insight of the different transfection methods and their parameters were obtained, which can ease the application of TGE.…

Kartoniert / Broschiert. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Aspoeck AndreasThe author studied biotechnology at the University of applied Life Science in Vienna, Austria. His main focus was engineering and lectures on animal cell culture. Upon completion of his work at the Department of Micr.…

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